Multiple molecular forms of Acanthamoeba lactic dehydrogenase

1. Acanthamoeba lactic dehydrogenase (D-lactate specific, EC 1.1.1.28) has been studied through the use of polyacrylamide gel electrophoresis (PAGE) and isoelectric focusing (PAGIEF). 2. Data from PAGE showed the presence of only a single macromolecular form of the enzyme in trophozoites, but PAGIEF data demonstrated that at least three LDH species occur. 3. Molecular weight determinations indicated a tetrameric assembly state for the enzyme. 4. Multiple molecular forms of the enzyme (isoenzymes) are encoded either by genes at two loci or by two alleles at a single LDH locus.     

A new mycotoxin from Fusarium

A new mycotoxin was isolated from a strain of Fusarium tricinctum cultivated for four months on a grain mixture. It was shown to be 4β, 8α-diacetoxy-12,13-epoxytrichotec-9-ene-3α, 15-diol by PMR and MS-analysis.     

Sesquiterpenoid stress compounds of the solanaceae

The sesquiterpenes isolated from species of the Solanaceae under various conditions of stress are reviewed, with brief references to other solanaceous stress metabolites. The chemistry and selected physical properties of the sesquiterpenoidal compounds are summarized with special emphasis on their ¹H and ¹³C NMR spectra. The close biogenetic relations between the compounds are discussed, and their possible biological function as antifungal agents (“phytoalexins”) is considered.